The South Gauteng High Court in Johannesburg has ruled in favour of the Canadian government's Export Development Canada (EDC) to ground the Guptas' private jet.
The whereabouts of the Bombadier Global 6000 jet is unknown, but the High Court in Johannesburg ordered that it must be landed and kept at the Lanseria airport outside the city.
The High Court has decided that the family's private jet must be handed over to EDC and stored at Lanseria Airport in Johannesburg.
The EDC, Canada's state-run trade credit agency, had together with Stoneriver, petitioned the court after the controversial family's businesses defaulted on paying its lease fees for the $41-million (33-million) plane.
The court ordered that the South African aviation authorities should cancel the plane's registration certificate if the family fails to comply with the order to return the aircraft.
EDC also had feared that the plane could be used to commit criminal offences given that its public tracking device had been switched off.
It further claimed it is concerned the Guptas may use the aircraft to escape justice or for some other unlawful means.
The three Gupta brothers are at the centre of graft and influence-peddling allegations surrounding Zuma, who was forced from office last month by the ruling African National Congress.