It is anticipated the jobs will be in place by the end of 2019.
USA based cyber security firm Anomali will create 120 new jobs with the opening of its European Research and Development Labs in Belfast.
Belfast has developed a significant cyber-security cluster with investments by firms like WhiteHat Security, Proofpoint and Black Duck Software.
Anomali already has operations in London and Berlin but its chief executive, Hugh Njemanze said it intends to make Belfast the base for "developing new products" and scaling its business in Europe and beyond.
"It is no surprise that Californian based Anomali has chosen to invest here based on our world-class credentials", continued Alastair.
Invest NI has said that the new jobs will contribute £4.1m in salaries for the Northern Irish economy and will help bolster its place as a centre for cybersecurity research and companies.
Commenting, Invest NI's CEO Alastair Hamilton said: "Northern Ireland is enjoying a growing worldwide reputation as a region of expertise and knowledge in cyber security".
Separately, Centre for Secure Information Technologies (CSIT) at Queen's University Belfast has said it is likely to secure £38.5 million of new research and development funding.
Northern Ireland is the number one location in the world for cyber security inward investment (FT fDI Markets 2013-16) and the number one global destination for U.S. cyber security companies with an eye for worldwide expansion.
CSIT, the UK's leading university centre for cyber security research, recently hosted its World Cyber Security Technology and Research Summit, which attracts worldwide cyber security experts and leaders from the world of industry, academia and government to Belfast each year.
"With the support of Invest NI, we have committed to making this strategic investment in Northern Ireland to drive innovation and support our global ambitions".